Publications | Department of Biology | University of Fribourg

C. De Virgilio publications

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2024	Caligaris M & De Virgilio C (2024) Proxies introduce bias in decoding TORC1 activity. microPubl. Biol. pdf Füllbrunn N, Nicastro R, Mari M, Griffith J, Herrmann E, Rasche R, Borchers AC, Auffarth K, Kümmel D, Reggiori F, De Virgilio C, Langemeyer L & Ungermann C. () (2024) The GTPase activating protein Gyp7 regulates Rab7/Ypt7 activity on late endosomes. J. Cell Biol. 223(6):e202305038. pdf
2023	Nicastro R, Brohée L, Alba J, Nüchel J, Figlia G, Kipschull S, Gollwitzer P, Romero-Pozuelo J, Fernandes SA, Lamprakis A, Vanni S (), Teleman AA (), De Virgilio C () & Demetriades C () (2023) Malonyl-CoA is a conserved endogenous ATP-competitive mTORC1 inhibitor. Nat. Cell Biol. 25, 1303-1318. pdf Caligaris M, Sampaio-Marques B, Hatakeyama R, Pillet B, Ludovico P, De Virgilio C, Winderickx J & Nicastro R. () (2023) The yeast protein kinase Sch9 functions as a central nutrient-responsive hub that calibrates metabolic and stress-related responses. J. Fungi 9, 787. pdf Grziwa S, Schäfer J-H, Nicastro R, Annabel A, De Virgilio C, Fröhlich F, Moeller A, Gao J, Langemeyer L () & Ungermann C () (2023) Yck3 casein kinase-mediated phosphorylation determines Ivy1 localization and function at endosomes and vacuole. J. Cell Sci. 136, jcs.260889. pdf Sambri S, Ferniani M, Campostrini G, Testa M, Meraviglia V, de Araujo M, Dokládal L, Vilardo C, Monfregola J, Zampelli N, Del Vecchio Blanco F, Torella A, Ruosi C, Fecarotta S, Parenti G, Staiano L, Bellin M, Huber L, De Virgilio C, Trepiccione F, Nigro N & Ballabio A () (2023). RagD auto-activating mutations impair MiT/TFE activity in kidney tubulopathy and cardiomyopathy syndrome. Nat. Commun. 14, 2775. pdf Deprez M-A, Caligaris M, Rosseels J, Hatakeyama R, Ghillebert R, Sampaio-Marques B, Mudholkar K, Eskes E, Meert E, Ungermann C, Ludovico P, Rospert S, De Virgilio C () & Winderickx J () (2023). The nutrient-responsive CDK Pho85 primes the Sch9 kinase for its activation by TORC1. PloS Genetics 19, e1010641. pdf Caligaris M, Nicastro R (), Hu Z, Tripodi F, Hummel JE, Deprez M-A, Winderickx J, Rospert S, Coccetti P, Dengjel J & De Virgilio C () (2022). Snf1/AMPK fine-tunes TORC1 signaling in response to glucose starvation. eLife 12, e84319. pdf
2022	Nicastro R, Gaillard H, Zarzuela L, Péli-Gulli MP, Fernández-García E, Tomé M, García-Rodríguez N, Durán RV & De Virgilio C () & Wellinger RE () (2022). Manganese is a physiologically relevant TORC1 activator in yeast and mammals. eLife 11, e80497. pdf Gao J, Nicastro R, Péli-Gulli MP, Grziwa S, Chen Z, Kurre R, Piehler J, De Virgilio C, Fröhlich F & Ungermann C. () (2022). The HOPS tethering complex is required to maintain the identity of signaling endosomes and TORC1 activity. J. Cell Biol. 221, e202109084. pdf
2021	Dokládal L, Stumpe M, Hu Z, Jaquenoud M, Dengjel J () & De Virgilio C () (2021). Phosphoproteomic responses of TORC1 target kinases reveal discrete and convergent mechanisms that orchestrate the quiescence program in yeast. Cell Rep. 37, 110149. pdf Nicastro R, Raucci S, Michel AH, Stumpe M, Garcia Osuna GM, Jaquenoud M, Kornmann M & De Virgilio C () (2021). Indole-3-acetic acid is a physiological inhibitor of TORC1 in yeast. PLoS Genetics 17, e1009414. pdfFaculty Opinions Dokládal L, Stumpe M, Pillet B, Hu Z, Garcia Osuna GM, Kressler D, Dengjel J & De Virgilio C () (2021). Global Phosphoproteomics pinpoints uncharted Gcn2-mediated mechanisms of translational control. Mol. Cell 81, 1879-1889.e6. pdfFaculty Opinions Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). (2021). Klionsky () et al. Autophagy 17, 1-382. pdf Tate JJ, Rai R, De Virgilio C, & Cooper, TG () (2021). N- and C-terminal Gln3-Tor1 interaction sites: one acting negatively and the other positively to regulate nuclear Gln3 localization. Genetics 217, iyab017. pdf
2020	Chen Z, Malia PC, Hatakeyama R, Nicastro R, Hu Z, Péli-Gulli MP, Gao J, Nishimura T, Eskes E, Stefan C, Winderickx J & De Virgilio C () & Ungermann C () (2021). TORC1 determines Fab1 lipid kinase function at signaling endosomes and vacuoles. Curr. Biol. 31, 1-13. pdf
2019	Brenna A, Olejniczak I, Chavan R, Ripperger JA, Langmesser S, Cameroni E, Hu Z, De Virgilio C, Dengjel J & and Albrecht U () (2019). Cyclin dependent kinase 5 (CDK5) regulates the circadian clock. eLife 8, e50925. pdf Zhang T, Péli-Gulli MP, Zhang Z, Tang X,, Ye J, De Virgilio C () & Ding J () (2019). Structural insights into the EGO-TC–mediated membrane tethering of the TORC1-regulatory Rag GTPases. Sci. Adv. 5, eaax8164. pdf Hu Z, Raucci S, Jaquenoud M, Hatakeyama R, Stumpe M, Rohr R, Reggiori F, De Virgilio C () & Dengjel J. () (2019). Multilayered control of protein turnover by TORC1 and Atg1. Cell Rep. 28, 3486-3496. pdf Kvainickas A, Nägele H, Qi W, Dokládal L, Jimenez-Orgaz A, Stehl L, Gangurde D, Zhao Q, Hu Z, Dengjel J, De Virgilio C, Baumeister R & Steinberg F () (2019). Retromer and TBC1D5 maintain late endosomal RAB7 domains to enable amino acid-induced mTORC1 signaling. J Cell Biol. 218, 3019-2038. pdf Hatakeyama R & De Virgilio C () (2019). TORC1 specifically inhibits microautophagy through ESCRT-0. Curr. Genet. 65, 1243-1249. pdf Hatakeyama R & De Virgilio C () (2019). A spatially and functionally distinct pool of TORC1 defines signaling endosomes in yeast. Autophagy 15, 915-916. pdf Hatakeyama R, Péli-Gulli MP, Hu Z, Jaquenoud M, Garcia Osuna GM, Sardu A, Dengjel J & De Virgilio C () (2019). Spatially distinct pools of TORC1 balance protein homeostasis. Mol. Cell 73, 325-338.e8. pdfFaculty Opinions
2018	Fruhmann G, Marchal C, Vignaud H, Verduyckt M, Talarek N, De Virgilio C, Winderickx J () & Cullin, C () (2018). The impact of ESCRT on Aß1-42 induced membrane lesions in a yeast model for Alzheimer’s disease. Front. Mol. Neurosci. 11, 409. pdf
2017	Péli-Gulli MP, Raucci S, Hu Z, Dengjel J & De Virgilio C () (2017). Feedback inhibition of the Rag GTPase GAP complex Lst4-Lst7 safeguards TORC1 from hyperactivation by amino acid signals. Cell Reports 20, 281-288. pdf Nicastro R, Sardu A, Panchaud N & De Virgilio C () (2017). The architecture of the Rag GTPase signaling network. Biomolecules 7(3), E48. pdf Wilms T, Swinnen E, Eskes E, Dolz-Edo L, Uwineza A, Van Essche R, Rosseels J, Zabrocki P, Cameroni E, Franssens V, De Virgilio C, Smits GJ & Winderickx J. () (2017). The yeast protein kinase Sch9 adjusts V-ATPase assembly/disassembly to control pH homeostasis and longevity in response to glucose availability. PLoS Genet. 13(6), e1006835. pdf Michel AH, Hatakeyama R, Kimmig P, Arter M, Peter M, Matos J, De Virgilio C & Kornmann B () (2017). Functional mapping of yeast genomes by saturated transposition. eLife 6, e23570. pdfFaculty Opinions Moreno-Torres M, Jaquenoud M, Péli-Gulli MP, Nicastro R & De Virgilio C () (2017). TORC1 coordinates the conversion of Sic1 from a target to an inhibitor of cyclin-CDK-Cks1. Cell Discovery 3, 17012. pdf
2016	Hatakeyama R & De Virgilio C () (2016). Unsolved mysteries of Rag GTPase signaling in yeast. Small GTPases 7, 239-246. pdf Powis K & De Virgilio C () (2016). Conserved regulators of Rag GTPases orchestrate amino acid-dependent TORC1 signaling. Cell Discovery 2, 15049. pdf
2015	Péli-Gulli M-P, Sardu A, Panchaud N, Raucci S & De Virgilio C () (2015). Amino acids stimulate TORC1 through Lst4-Lst7, a GTPase activating protein complex for the Rag family GTPase Gtr2. Cell Rep. 13, 1-7. pdf Moreno-Torres M, Jaquenoud M & De Virgilio C () (2015). TORC1 controls G1–S cell cycle transition in yeast via Mpk1 and the greatwall kinase pathway. Nat. Commun. 6, 8256. pdf Numrich J, Péli-Gulli M-P, Arlt H, Sardu A, Griffith J, Levine T, Engelbrecht-Vandré S, Reggiori F, De Virgilio C & Ungermann C () (2015). The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis. J. Cell Sci. 128, 2278-2292. pdf Powis K, Zhang T, Panchaud N, Wang R, De Virgilio C () & Ding J () (2015). Crystal structure of the Ego1-Ego2-Ego3 complex and its role in promoting Rag GTPase-dependent TORC1 signaling. Cell Res. 25, 1043-1059. pdf
2014	Dubots E, Cottier S, Péli-Gulli MP, Jaquenoud M, Bontron S, Schneiter R & De Virgilio C () (2014). TORC1 regulates Pah1 phosphatidate phosphatase activity via the Nem1/Spo7 protein phosphatase complex. PLoS ONE 9, e104194. pdf
2013	Panchaud N, Péli-Gulli MP & De Virgilio C () (2013). SEACing the GAP that nEGOCiates TORC1 activation: Evolutionary conservation of Rag GTPase regulation. Cell Cycle 12, 2948-2952. pdf Talarek N, Bontron S & De Virgilio C () (2013). Quantification of mRNA stability of stress-responsive genes following conditional excision of open reading frames. RNA Biol. 10, 1299-1306. pdf Panchaud N, Péli-Gulli MP & De Virgilio C () (2013). Amino acid deprivation inhibits TORC1 through a GTPase-activating protein complex for the Rag family GTPase Gtr1. Sci. Signal. 6, ra42. pdfFaculty Opinions Bontron S, Jaquenoud M, Vaga S, Talarek N, Bodenmiller B, Aebersold R. & De Virgilio C. () (2013). Yeast endosulfines control entry into quiescence and chronological life span by inhibiting protein phosphatase 2A. Cell Rep. 3, 16-22. pdf
2012	Zhang T, Péli-Gulli MP, Yang H, De Virgilio C () & Ding, J. () (2012). Ego3 functions as a homodimer to mediate the interaction between Gtr1-Gtr2 and Ego1 in the EGO complex to activate TORC1. Structure 20, 2151-2160. pdf Bonfils G, Jaquenoud M, Bontron S, Ostrowicz C, Ungermann C & De Virgilio C () (2012). Leucyl-tRNA synthetase controls TORC1 via the EGO complex. Mol. Cell 46, 105-110. pdf Chen J (), Young SM, Allen C, Seeber A, Péli-Gulli MP, Panchaud N, Waller A, Ursu O, Yao T, Golden J, Strouse JJ, Carter MB, Kang H, Foutz T, Bologa CG, Edwards B, Peterson BR, Aubé J, Werner-Washburne M, Loewith R, De Virgilio C & Sklar LA () (2012). Identification of a small molecule yeast TORC1 inhibitor with a flow cytometry based multiplex screen. ACS Chem. Biol. 7, 715-722. pdf De Virgilio C () (2012). The essence of yeast quiescence. FEMS Microbiol. Rev. 36, 306-339. pdf
2011	Kawai S (), Urban J, Panchaud N, De Virgilio C & Loewith R () (2011). Mitochondrial genomic dysfunction causes dephosphorylation of Sch9 in the yeast Saccharomyces cerevisiae. Eukaryot. Cell 10, 1367-1369. pdf Luo X, Talarek N & De Virgilio C () (2011). Initiation of the yeast G0 program requires Igo1 and Igo2, which antagonize activation of decapping of specific nutrient-regulated mRNAs. RNA Biol. 8, 14-17. pdf
2010	Talarek N, Cameron E, Jaquenoud M, Luo X, Bontron S, Lippman S, Devgan G, Snyder M, Broach JR & De Virgilio C () (2010). Initiation of the TORC1-regulated G0 program requires Igo1/2, which license specific mRNAs to evade degradation via the 5'-3' mRNA decay pathway. Mol. Cell 38, 345-355. pdf Faculty Opinions Mok J, Kim PM, LaM HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A, Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein MB, Snyder M () & Turk BE () (2010). Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs. Sci. Signal. 3, ra12. pdfFaculty Opinions Binda M, Bonfils G, Panchaud N, Péli-Gulli MP & De Virgilio C () (2010). An EGOcentric view of TORC1 signaling. Cell Cycle 9, 221-222. pdf Smets B, Ghillebert R, De Snijder P, Binda M, Swinnen E, De Virgilio C & Winderickx J () (2010). Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae. Curr. Genet. 56, 1-32. pdf
2009	Binda M., Péli-Gulli MP, Bonfils G, Panchaud N, Urban J, Sturgill TW, Loewith R & De Virgilio C () (2009). The Vam6 GEF controls TORC1 by activating the EGO complex. Mol. Cell 35, 563-573. pdf Faculty Opinions
2008	Zabrocki P, Bastiaens I, Delay C, Bammens T, Ghillebert R, Pellens K, De Virgilio C, Van Leuven F & Winderickx J () (2008). Phosphorylation, lipid raft interaction and traffic of α-synuclein in a yeast model for Parkinson. Biochim. Biophys. Acta 1783, 1767-1780. pdf Wanke V, Cameroni E, Uotila A, Piccolis M, Urban J, Loewith R () & De Virgilio C () (2008) Caffeine extends yeast life span by targeting TORC1. Mol. Microbiol. 69, 277-285. pdfFaculty Opinions Fiechter V, Cameroni E, Cerutti L, De Virgilio C, Barral Y & Fankhauser C () (2008). The evolutionary conserved BER1 gene is involved in microtubule stability in yeast. Curr. Genet. 53, 107-115. pdf Froquet R, Cherix N, Birke R, Benghezal M, Cameroni E, Letourneur F, Mösch HU, De Virgilio C & Cosson P () (2008). Control of cellular physiology by TM9 proteins in yeast and Dictyostelium. J. Biol. Chem. 283, 6764-6772. pdf
2007	Mulder KW, Inagaki A, CameronI E, Mousson F, Winkler GS, De Virgilio C, Collart MA & Timmers HTM () (2007). Modulation of Ubc4p/Ubc5p-mediated stress responses by the RING-finger dependent ubiquitin-protein ligase Not4p in Saccharomyces cerevisiae. Genetics 176, 181-192. pdf De Filippi L, Fournier M, Cameroni E, Linder P, De Virgilio C, Foti M & Deloche O () (2007). Membrane stress is coupled to a rapid translational control of gene expression in chlorpromazine-treated cells. Curr. Genetics 52, 171-185. pdf Urban J, Soulard A, Huber A, Lippman S, Mukhopadhyay D, Deloche O, Wanke V, Anrather D, Ammerer G, Riezman H, Broach JR, De Virgilio C. Hall MN & Loewith R () (2007). Sch9 is a major target of TORC1 in Saccharomyces cerevisiae. Mol. Cell. 26, 663-667. pdfFaculty Opinions
2006	Cameroni E, De Virgilio C & Deloche O () (2006). The phosphatidylinositol 4-phosphate is required for protein translation initiation in Saccharomyces cerevisiae. J. Biol. Chem. 281: 38139-38149. pdf De Virgilio C () & Loewith R () (2006) Cell growth control: little eukaryotes make big contributions. Oncogene 25, 6392-6415. pdf De Virgilio C () & Loewith R () (2006) The TOR signalling network from yeast to man. Int. J. Biochem. & Cell Biol. 38, 1476-1481. pdf Swinnen E, Wanke V, Roosen J, Smets B, Pedruzzi I, Cameroni E, De Virgilio C & Winderickx J () (2006) Rim15 and the crossroads of nutrient signalling in Saccharomyces cerevisiae. Cell Div. 1, 3. pdf
2005	Wanke V, Pedruzzi I, Cameroni E, Dubouloz F & De Virgilio C () (2005). Regulation of G0 entry by the Pho80-Pho85 cyclin-CDK complex. EMBO J. 24, 4271-4278. pdf Ptacek J, Devgan G, Michaud G, Zhu H., Zhu X, Fasolo J, Guo H, Jona G, Breitkreutz A, Sopko R, Lee SJ, McCartney RR, Schmidt MC, Rachidi N, Stark MJR, Stern DF, De Virgilio C, Tyers M, Andrews B, Gerstein M, Schweitzer B, Predki PF & Snyder M () (2005). Global analysis of protein phosphorylation in yeast. Nature 438, 679-684. pdfFaculty Opinions Knaus M, Cameroni E, Pedruzzi I, Tatchell K, De Virgilio C() & Peter, M. () (2005). The Bud14p-Glc7p complex functions as a cortical regulator of dynein in budding yeast. EMBO J. 24, 3000-3011. pdf Dubouloz F, Deloche O, Wanke V, Cameroni E & De Virgilio C () (2005). The TOR and EGO protein complexes orchestrate microautophagy in yeast. Mol. Cell 19, 15-26. pdfFaculty Opinions Roosen J, Engelen K, Marchal K, Mathys J, Griffioen G, Cameroni E, Thevelein JM, De Virgilio C, De Moor B & Winderickx J () (2005). PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability. Mol. Microbiol. 55, 862-880. pdf Lenssen E, James N, Pedruzzi I, Dubouloz F, Cameroni E, Bisig R, Maillet L, Werner M, Roosen J, Petrovic K, Winderickx J, Collart MA () & De Virgilio C () (2005). The Ccr4-Not complex independently controls both Msn2-dependent transcriptional activation – via a newly identified Glc7/Bud14 type I protein phosphatase module – and TFIID promoter distribution. Mol. Cell. Biol. 25, 488-498. pdf
2004	Cameroni E., Hulo N, Roosen J, Winderickx J & De Virgilio C () (2004). The novel yeast PAS kinase Rim15 orchestrates G0-associated antioxidant defense mechanisms. Cell Cycle 3, 462-468. pdf
2003	Pedruzzi I, Dubouloz F, Cameroni E, Wanke V, Roosen J, Winderickx J & De Virgilio C () (2003). TOR and PKA signaling pathways converge on the protein kinase Rim15 to control entry into G0. Mol. Cell 12,1607-1613. pdf Faculty Opinions
2002	Lee PR, Song S, Ro HS, Park CJ, Lippincott J, Li R, Pringle JR, De Virgilio C, Longtine MS & Lee KS () (2002). Bni5p, a septin-interacting protein, is required for normal septin functionand cytokinesis in S. cerevisiae. Mol. Cell. Biol. 22, 6906-6920. pdf Zaragoza O, De Virgilio C, Ponton J & Gancedo C () (2002). Disruption in Candida albicans of the TPS2 gene encoding trehalose-6-phosphate phosphatase affects cell integrity and decreases infectivity. Microbiol. 148, 1281-1290. pdf Lenssen E, Oberholzer U, Labarre J, De Virgilio C. & Collart MA () (2002). Saccharomyces cerevisiae Ccr4-Not complex contributes to the control of Msn2-dependent transcription by the Ras/cAMP pathway. Mol. Microbiol. 43, 1023-1037. pdf
2001	Harkins HA, Pagé N, Schenkman LR, De Virgilio C, Shaw S, Bussey H & Pringle JR () (2001). Bud8p and Bud9p, proteins that may mark the sites for bipolar budding in yeast. Mol. Biol. Cell 12, 2497-2518. pdf
2000	Pedruzzi I, Bürckert N, Egger P & De Virgilio C () (2000). Saccharomyces cerevisiae Ras/cAMP pathway controls post-diauxic shift element-dependent transcription through the zinc finger protein Gis1. EMBO J. 19, 2569-2597. pdf
1999	Reinders A, Romano I, Wiemken A. & De Virgilio C () (1999). The thermophilic yeast Hansenula polymorpha does not require trehalose synthesis for growth at high temperatures but does for normal acquisition of thermotolerance. J. Bacteriol. 181, 4665-4668. pdf
1998	Bell W, Sun W, Hohmann S, Wera S, Reinders A, De Virgilio C, Wiemken A & Thevelein J () (1998). Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex. J. Biol. Chem. 273, 33311-33319. pdf Hochstrasser U, Lüscher M, De Virgilio C, Boller T & Wiemken A () (1998). Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris. FEBS Lett. 440, 356-360. pdf Reinders A, Bürckert N, Boller T, Wiemken A & De Virgilio C () (1998). Saccharomyces cerevisiae cAMP-dependent protein kinase controls entry into stationary phase through the Rim15p protein kinase. Genes & Dev. 12, 2943-2955. pdf
1997	DeMarini DJ, Adams AEM, Fares H, De Virgilio C, Valle G, Chuang JS & Pringle JR () (1997). A septin-based hierachy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiae cell wall. J. Cell Biol. 139, 75-93. pdf Ribeiro MJS, Reinders A, Boller T. Wiemken A & De Virgilio C () (1997). Trehalose synthesis is important for the acquisition of thermotolerance in Schizosaccharomyces pombe. Mol. Microbiol. 25, 571-582. pdf Reinders A, Bürckert N, Hohmann S, Thevelein JM, Boller, Wiemken A & De Virgilio C () (1997). Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock. Mol. Microbiol. 24, 687-695. pdf
1996	De Virgilio C, DeMarini DJ & Pringle JR () (1996). SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells. Microbiol. 142, 2897-2905. pdf Longtine MS., DeMarini DJ, Valencik ML, Al-Awar OS, Fares H, De Virgilio C. & Pringle JR () (1996). The septins: roles in cytokinesis and other processes. Curr. Opin. Cell. Biol. 8, 106-119. pdf
1995	Pringle JR (), Bi E, Harkins HA, Zahner J, De Virgilio C, Chant J, Corrado K & Fares H (1995). Establishment of cell polarity in yeast. Cold Spring Harbor Symp. Quant. Biol. 60, 729-744. pdf Stevenson BJ, Ferguson B, De Virgilio C, Bi E, Pringl JR, Ammerer G & Sprague Jr. GF () (1995). Mutation of RGA1, which encodes a putative GTPase-activating protein for the polarity-establishment protein Cdc42p, activates the pheromone-response pathway in the yeast Saccharomyces cerevisiae. Genes & Dev. 9, 2949-2963. pdf Simon MN, De Virgilio C, Souza B, Pringle JR, Abo A & Reed SI () (1995). Role for the Rho-family GTPase Cdc42p in yeast mating pheromone signal pathway. Nature 376, 702-705. pdf Cvrcková F, De Virgilio C, Manser E, Pringle JR & Nasmyth K () (1995). Ste20-like protein kinases are required for localized cell growth and cytokinesis in budding yeast. Genes & Dev. 9, 1817-1830. pdf
1994	Hottiger T (), De Virgilio C, Hall MN, Boller T & Wiemken A (1994). The role of trehalose synthesis for the acquisition of thermotolerance in yeast. II. Physiological concentrations of trehalose increase the thermal stability of proteins in vitro. Eur. J. Biochem. 219, 187-193. pdf De Virgilio C, Hottiger T, Dominguez J, Boller T. & Wiemken A () (1994). The role of trehalose synthesis for the acquisition of thermotolerance in yeast. I. Genetic evidence that trehalose is a thermoprotectant. Eur. J. Biochem. 219, 179-186. pdf
1993	Steensma HY, Barth G & De Virgilio C(1993). Genetic and physical localization of the acetyl-coenzyme A ynthetase gene ACS1, on chromosome I of Saccharomyces cerevisiae. Yeast 9, 419-421. pdf De Virgilio C, Bürckert N, Bell W, Boller T & Wiemken A () (1993). Disruption of TPS2, the gene encoding the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity. Eur. J. Biochem. 212, 315-323. pdf De Virgilio C, Bürckert N, Neuhaus, JM, Boller T & Wiemken A () (1993). CNE1, a Saccharomyces cerevisiae homologue of the genes encoding mammalian calnexin and calreticulin. Yeast 9, 185-188. pdf
1992	De Virgilio C, Bürckert N, Barth G, Neuhaus JM, Boller T & Wiemken A () (1992). Cloning and disruption of a gene required for growth on acetate but not on ethanol: the acetyl-coenzyme A synthetase gene of Saccharomyces cerevisiae. Yeast 8, 1043-1051. pdf Hottiger T, De Virgilio C, Bell W, Boller T & Wiemken A () (1992). The 70-kilodalton heat-shock proteins of the SSA subfamily negatively modulate heat-shock-induced accumulation of trehalose and promote recovery from heat stress in the yeast, Saccharomyces cerevisiae. Eur. J. Biochem. 210, 125-132. pdf
1991	De Virgilio C, Bürckert N, Boller T & Wiemken A () (1991). A method to study the rapid phosphorylation-related modulation of neutral trehalase activity by temperature shifts in yeast. FEBS Lett. 291, 355-358. pdf De Virgilio C, Piper P, Boller T & Wiemken A () (1991). Acquisition of thermotolerance in Saccharomyces cerevisiae without heat shock protein hsp104 and in the absence of protein synthesis. FEBS Lett. 288, 86-90. pdf De Virgilio C, Müller J, Boller T & Wiemken A () (1991). A constitutive, heat shock-activated neutral trehalase occurs in Schizosaccharomyces pombe in addition to the sporulation-specific acid trehalase. FEMS Microbiol. Lett. 84, 85-90. pdf
1990	De Virgilio C, Simmen U., Hottiger T, Boller T & Wiemken A () (1990). Heat shock induces enzymes of trehalose metabolism, trehalose accumulation, and thermotolerance in Schizosaccharomyces pombe, even in the presence of cycloheximide. FEBS Lett. 273, 107-110. pdf