Leica TCS SP5 (Bio)

Leica_TCS_SP5_Bio

The TCS SP5 is an upright confocal laser scanning microscope suitable for:

Fixed tissue imaging
- Live cell imaging
- z-stack
- Time lapse
- Tile scan
- ROI scan

 

Key features

- Upright DM6000CS stand with motorised stage
- Super-z Galvo
- 3 PMT detectors, 1 transmitted light detector
- Tunable emission filters
- Widefield fluorescent cubes: GFP, Rhodamine, DAPI
- Contrast method: DIC
- EL6000 Light Source
- Software Leica LAS (Version 2.7.3)

 

Objectives

FLATFIELD CORRECTION ABERRATION CORRECTION MAG. NA IMMERSION COVERGLASS
CONTRAST OTHER
Plan FLUOTAR 10 0.3 Dry 0.17
Plan APO lambda blue 20 0.7 Glyc/Water 0.17 DIC C Corr. lambda blue
Corr. UV
Plan APO 40 1.3 Oil 0.17 DIC D Corr. CS
Corr. UV
Plan APO CS 63 1.3 Glyc 0.14-0.20 DIC D Corr. UV
Plan APO lambda blue 63 1.3 Water 0.14-0.20 DIC D Corr. lambda blue
Corr. UV

The DIC is setup for the 20x and 63x

Lasers

Diode Laser: 405 nm
Argon Laser: 458nm, 476nm, 488nm, 514nm
Helium-Neon Laser: 543
Helium-Neon Laser: 633

light spectrum

 

Filters (widefield)

POSITION FILTER CUBE EXCITATION RANGE EXcITATION FILTER DICHROMATIC MIRROR SUPPRESSION FILTER
1 I3 Blue BP 450-490 510 LP 515
2 N2.1 Green BP 515-560 580 LP 590
3 Ana - - - Analyzer
4 A UV BP 340-380 400 LP 425

Read up about filter-cubes.
Use the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.

Motorized stage

Motorised Scanning stage for DM6000CS