General

The minimal delay between starting up and shutting down the microscope is 30 minutes! You also need to wait 30 minutes before starting again after shut down.
Only glycerol or water immersion but no immersion oil should be used on this system
If somebody uses the microscope after you, follow the standby protocol
Do not use your home folder (c:\users\<you login>) to save acquired data. Use D:\User Data\ for image data. Beware that this is no place to store data for longterm. We clean image data regularly.
Save your data on your external memory stick or harddrive

Start Protocol

switch on the three green buttons on the main switch board: PC/Microscope, Scanner Power and Laser Power
turn the Laser Key to the position ON
turn on the Epifluorescent Light Source
log in to your account
double click on LAS AF programme icon
when asked specify configuration to machine and DM6000
initialize microscope stand
ready to go!

If someone will use the microscope after you within the next 1-2 hours:

in the LAS AF software go to the configuration panel and decrease the laser power to 0% (no need to tick off the laser check boxes)
exit the LAS AF software
log off the computer
clean the objectives

Transfer your data on an external saving device since data on the PC computer will regularly be deleted.

in the LAS AF configuration window, decrease the laser power to 0% (no need to tick off the laser boxes)
close the LAS AF software
log out from your account
turn off the Epifluorescent Light Source
switch off the PC/Microscope button
switch off the Scanner Power button
turn the Laser Key to the position OFF
leave the Laser Power button on to allow the laser to cool down for at least 20 minutes!!!
In the meantime:
- clean up your stuff
- clean the objectives
- put the cover on the microscope
- make sure the immersion liquid bottles are closed properly.
after 20 minutes, when the laser fan shuts down (and you can hear yourself again), switch of the Laser Power button