Leica TCS SPE-II (Bio)

Leica_TCS_SPEII_Bio

The TCS SPE-II is an upright confocal laser scanning microscope suitable for:

- Fixed tissue imaging
- z-stack
- Tile scan
- ROI scan

 

Key features

- Upright DM5500Q stand with motorised stage
- Super-z Galvo
- 1 PMT detector
- Tunable emission filters
- Widefield fluorescent cubes: GFP, Rhodamine, DAPI
- EL6000 Light Source
- Software Leica LAS (Version x.x.x)

 

Objectives

ABERRATION CORRECTION Magnification NA Immersion CoverGLASS Other
APO 10 0.3 Dry 0.17
APO 20 0.6 Oil 0.17 Imm. CS
APO 40 1.15 Oil 0.17 CS
APO 63 1.3 Oil 0.17 CS

 

Lasers

Diode Laser: 405 nm
Diode Laser: 488nm
Diode Laser: 532nm
Diode Laser: 635nm

light spectrum

 

Filters (widefield)

POSITION FILTER CUBE EXCITATION RANGE EXITATION FILTER DICHROMATIC MIRROR SUPPRESSION FILTER
1 I3 Blue BP 450-490 510 LP 515
2 N2.1 Green BP 515-560 580 LP 590
3 A UV BP 340-380 400 LP 425

Read up about filter-cubes.
Use the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.

Motorized stage

Motorised Scanning stage for DM5500Q