The TCS SPE-II is an upright confocal laser scanning microscope suitable for:
- Fixed tissue imaging
- z-stack
- Tile scan
- ROI scan
Key features
- Upright DM5500Q stand with motorised stage
- Super-z Galvo
- 1 PMT detector
- Tunable emission filters
- Widefield fluorescent cubes: GFP, Rhodamine, DAPI
- EL6000 Light Source
- Software Leica LAS (Version x.x.x)
Objectives
| ABERRATION CORRECTION | Magnification | NA | Immersion | CoverGLASS | Other |
|---|---|---|---|---|---|
| APO | 10 | 0.3 | Dry | 0.17 | |
| APO | 20 | 0.6 | Oil | 0.17 | Imm. CS |
| APO | 40 | 1.15 | Oil | 0.17 | CS |
| APO | 63 | 1.3 | Oil | 0.17 | CS |
Lasers
Diode Laser: 405 nm
Diode Laser: 488nm
Diode Laser: 532nm
Diode Laser: 635nm
Filters (observation with binocular)
| POSITION | FILTER CUBE | EXCITATION RANGE | EXITATION FILTER | DICHROMATIC MIRROR | SUPPRESSION FILTER |
|---|---|---|---|---|---|
| 1 | I3 | Blue | BP 450-490 | 510 | LP 515 |
| 2 | N2.1 | Green | BP 515-560 | 580 | LP 590 |
| 3 | A | UV | BP 340-380 | 400 | LP 425 |
Read up about filter-cubes.
Use the FPbase or the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.
Motorized stage
Motorised Scanning stage for DM5500Q