GE DeltaVision Elite (Med)

ge_deltavision_elite

The DeltaVision Elite from Applied Precision is an inverted wide-filed fluorescence microscope for live cell imaging.

 

Key features

- Olympus IX71 inverted microscope
- CoolSnap HQ2 cooled CCD camera
- Fast excitation switching
- Laser module for TIRF/FRAP (The objectives currently only support the FRAP applicaiton)
- Motorized stage and focus
- Hardware based autofocus
- Incubation Chamber (C02, himidity and temperature control)
- On-site Deconvolution

 

Objectives

all objectives are from Olympus

FLATFIELD CORRECTION ABERRATION CORRECTION MAG. NA IMMERSION COVER GLASS
CONTRAST OTHER
U Plan S Apo 20 0.75 air 0.17
U Plan FL 40 1.3 oil 0.17
Plan Apo N 60 1.42 oil 0.17
U Plan S Apo 100 1.40 oil 0.17

 

Lasers

Diode Laser: 405 nm (100mW), 488nm (50mW), 514nm (50mW), 561nm (50mW) e

light spectrum

The lasers can be used for FRAP. TIRF is not possible since we currently do not have the necessary objective.

Filters

Read up about filter-cubes.
Use the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.

Excitation

DAPI, FITC, TRITC, Cy5, POL

Emission

Blank, DAPI, FITC, TRITC, Cy5

Polychroic

(non motorized turret)

POSITION FILTER CUBE Reflection bands
Transmission bands
1 quad (DAPI/FITC/TRIC/Cy5) 381-401, 464-492,
531-556, 619-644
409-456, 500-523, 564-611, 652-700
2 quad-mch (DAPI/FITC/mCh/Cy5) 381-401, 464-492, 561-590, 625-644 409-456, 500-553, 598-617, 652-700
3 CFP/YFP 400-454, 496-528 463-487, 537-559
4 GFP/mCh 425-495, 555-590 500-550, 600-675
5 CFP/YFP/mCh 400-454, 496-528, 558-593 463-487, 537-550, 602-648

 

Motorized stage

Property X,Y Z
Accuracy < 10.0 um per 25 mm < 0.6 um per 13 um
Repeatability < ± 0.2 um < ± 0.1 um
Step resolution 20 nm 5 nm
Maximum travel 25 mm x 50 mm 1 mm