Visitron VisiScope CSU-W1

Visitron VisiScope CSU-W1

The VisiScope CSU-W1 is an inverted Spinning Disk Confocal Microscope assembled by Visitron System GmbH

 

Key features

- Nikon Ti-E inverted microscope
- Hardware autofocus (Perfect Focus)
- Motorized stage
- CSU-W1 Spinning Disk Head with 50um pinhole disk
- Brightfield bypass option
- Evolve 512 (Photometrics) EM-CDD camera (16um pixel size)
- Scientific Grade 4.2 sCMOS camera: (6.5 um)
- Small environmental sample chamber (temperature control, CO2 control)
- Photomanipulation module (FRAP, FRET, Photoactivation)
- Laser ablation module

 

Objectives

all objectives are from Nikon’s CFI series

FLATFIELD CORRECTION ABERRATION CORRECTION MAG. NA IMMERSION COVERGLASS
CONTRAST OTHER
Achromat 10 0.25 Dry PH1
Plan Apochormat 20 0.75 Dry 0.17 DIC, PH2 lambdaDM
Apochromat 40 1.15 Water 0.15-0.19 DIC, PH3 LWD lambdaS
Plan Apochromat 60 1.25 Water 0.15-0.18 DIC, PH3 VC A
Plan Apochromat 100 1.3 Oil 0.17 DIC, PH3 VC

Lasers

Solid State Laser:
405nm (120mW)
445nm (100mW)
488nm (200mW)
515nm (100mW)
561nm (200mW)

light spectrum

 

Filters (widefield)

POSITION FILTER CUBE EXCITATION RANGE EXcITATION FILTER Dichroic Beamsplitter Emission FILTER
1  Brightfield
2  DIC
3  DAPI  320-520 D350/50x  T400LP  ET460/50m
4 GFP  400-700  ET470/40x  T495LPXR  ET525/50m
5 mCherry  500-700  ET560/40x  T585lp  ET630/75m

Read up about filter-cubes.
Use the FPbase or the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.

 

Filters (EM-CCD camera)

POSITION FILTER WheEL Single Band Emission
1  Brightfield
2  DAPI 460/50
3  GFP 525/50
4  mCherry 609/57
5  mRFP 575LP
6  CFP 470/24
7  YFP 535/30
8  GFP 510/20

 

 

Filters (sCMOS camera)

POSITION FILTER WHEEL Single Band Emission
1  Brightfield
2  DAPI 460/50
3  GFP 525/50
4  mCherry 609/57
5  mRFP/mCherry 575LP
6  CFP 470/24
7 YFP 535/30
8 GFP 510/20

Motorized stage

-travel range 120mm x 100mm
-resolution: 0.05 micrometer
-repeatability: 0.75 micrometer
-accuracy: 6 micrometer
-speed: 600mm/sec

Piezo Z-microscope stage insert
-travel range 350 micrometer
-resolution about 3 nanometer
-repeatability 1 nanometer