The Hamamatsu NanoZoomer S60 is a fully automated whole slide scanner.
Key Features
- Fully automated multi-slide scanning system
- Orca Flash 4.0 (C11440-42U30) sCMOS camera
- Wide-field color imaging
- Epi-fluorescence monochrome imaging
- 60/30 capacity of standard/double slides
- FL-illumination lamp unit for S60 L13820-03
Slide Formats
| Slide Width | Slide Height | Thickness | Max. Load | |
|---|---|---|---|---|
| Standard | 26 mm | 76 mm | 0.9-1.2 mm | 60 slides |
| Double | 52 mm | 76 mm | 0.9-1.2 mm | 30 slides |
Scanning Modes
| Magnification | NA | Coverglass | Scan speed | Resolution |
|---|---|---|---|---|
| 20x | 0.75 | 0.17 | 3.75 mm2 s | 0.46 um pixel |
| 40x (2x20x) | 0.75 | 0.17 | 1.5 mm2 s | 0.23 um pixel |
Fluorescence
Read up about filter-cubes.
Use the FPbase or the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.
Filtersets
filter sets comprise a excitation filter wheel position, a filter cube and a emission filter wheel position.
| Filter SET | Excitation filter | Ex . Pos. | Dichroic mirror | Cube # | Suppression filter | Em. Pos. |
|---|---|---|---|---|---|---|
| DAPI | 387/11 | 1 | 408 | 1 | 440/40 | 1 |
| FITC/Alexa488 | 485/20 | 2 | 504 | 1 | 525/30 | 2 |
| Cy3/Alexa555 | 534/40, 535/50 | 5 | 560 | 2 | 577/28, 585/40 | 6 |
| Cy3.5 | 560/25 | 3 | 581 | 1 | 607/36 | 3 |
| Cy5/Alexa647 | 635/36, 594LP | 6 | 659 | 2 | 681/55, 681/24 | 5 |
| Cy5.5 | 655/40, 594LP | 6 | 685 | 3 | 716/40, 681/24 | 5 |
| Cy7 | 740/13 | 4 | 762 | 1 | 809/81 | 4 |
| Dihydroethidium | 485/20 | 2 | 504, 581 | 1 | 607/36 | 3 |
Filter Cube
| Cube # | Name | Excitation | Dichroic | Emission |
|---|---|---|---|---|
| 1 | Multi-dichroic | - | 408, 504, 581, 667, 762 | - |
| 2 | Cy3/Cy5 | 534/40, 635/36 | 560, 659 | 577/28, 690/50 |
| 3 | Cy5.5-C | 655/40 | 685 | 716/40 |