All posts by Felix Meyenhofer

HuPro44_2

Huygens Professional

Our image processing workstation is now equipped with Huygens Professional (desktop). Compared to our Huygens Remote Manager (HRM) the HuPro allows for more flexibility and provides some additional functionality.

The new HuPro desktop version of the Huygens software is intended to accelerate the optimization of the deconvolution parameter and do advanced image restoration using measured point spread functions (in contrast to the theoretical PSF).

This new license replaces the Huygens Essential that was previously accessible via remote desktop connection.

imaris-filament-example

Imaris 9.3

Imaris 9.3 is available. Find out more about the software on the Bitplane website.

The first apparent change will be the end of the arena. Instead of its own database, there is now the possibility to add ‘observed’ folders to Imaris to facilitate the access to your ims-files.
The first time Imaris is launched, you will be prompted to use the migration assistant, that allows to extract existing images from the arena. You can find a short tutorial in this video.

You might also wonder look in vain for functionality like filtering or background subtraction under  Menu > Image Processing. Now instead there is a new icon in the toolbar:

imaris-ip-icon

This will bring up the following window where you can chain together all the basic pre-processing operations together for the different channels of your dataset.

imaris-ip-window

The complete list of novelties can be found on the ‘what’s new‘ page. You might also be interested in the change-list in the release notes.

We installed this latest version on our number crunchers.

fp-base-screenshot

FPbase

fpbase.org is a beautiful web-application to browse the contents of the Fluorescent Protein Database. There is a lot of linked content such as excitation and emission spectra or the publications reporting on the molecules. Check it out!

leica_flim-example-2

Demo: Leia SP8 FALCON

leica_dmi8

At the beginning of this year we will continue the demo series for the laser scanning confocal system. Leica will bring their SP8 FALCON system for a week.

Dates: 11-20 January 2019
Location: PER017, 013

Key Features

  • Inverted microscope (DMi8)
  • Fully motorised
  • White Light Laser (WLL)
  • Acousto-Optical Beam-Splitter (AOBS) 470-670
  • 405 Diode
  • Hybrid detectors HyD
  • FLIM module FALCON
  • Simultaneous multiple colors and super-resolution mode LIGHNING
  • Stage application module NAVIGATOR

 

Objectives

FLATFIELD CORRECTION Chromatic CORRECTION MAG. NA IMMERSION Remarks
Plan FLUOTAR 4 0.13 Dry
Plan Apochromat 10 0.45 Dry CS2
Plan Apochromat 20 0.75 Dry CS2
Plan Apochromat 40 1.10 Water CS2 motCORR
Plan Apochromat 63 1.4 Oil CS2
Plan Apochromat 63 1.3 Glyc. CORR CS2

Schedule

dates description
14.-15. Jan. Installation of the system
16.-17 Jan. Demo by Nathalie Garin.
People with samples can reserve 1 hour slots.
For 1 hour demos without your own samples, please organise in groups of 1-4 people.
18. Jan. Further testing (reserve)
21. Jan. Dismounting of the system

 

Demo-Slot Booking

To reserve a slot for the demo please login.

imaris-track-example

Imaris Workshop 11. September 2018

At the University of Fribourg we use Imaris to process and analyse multidimensional image data-sets issue from epi-fluorescene microscopy.  In particular we use it’s advanced functionality for 3 dimensional (optically sectioned) image data and perform volumetric analysis.

We organise a worksop with representatives of Bitplane for you, to discover the possibilities of the software and train yourself as a user.

Further information:

Organisation

date: 11. September 2018 from 9:00-17:00
location: PER II, F230
participation conditions: UniFr staff, free of charge, registration required

Participants are free to only attend the presentation of Imaris or to work through the entire workshop.

Schedule

TIME DESCRIPTION
 09:00-10:30 presentation of Imaris (functionality, use cases)
 10:30-10:45   coffee break
 11:00-13:00 introduction to Imaris (basics)
13:00-14:00   lunch break
14:00-15:30 hands-on workshop
15:30-16:00   coffee break
16:00-17:30 hands-on workshop

 

Registration

registration closed.

Usage Rules

  1. All facility users have to take a training session with facility staff for any given instrument they intend to use (contact).
  2. Every user books his/her own slots.
  3. During office hours – Monday to Friday from 9:00 to 17:00 – the following applies:
    1. An oh-slot (office hour slot)  has, for counting purposes, a max. duration of 4 hours. E.g.  single oh-slots are 9:00-13:00, 13:00-17:00 but also 7:00-12:00. A reservation from 8:00- 13:30 would count as two oh-slots.
    2. At any given time you are allowed max. three upcoming oh-slots.
    3. If possible avoid several slots during office hours on one single day.
  4. Outside of the office hours there is no limitation for the duration of the reservation slots.
  1. Cancellation must be done before your booked slot has started. Once the slot started you will be charged for the full slot.
  2. If you finish your session earlier than booked, please let the next user know or by letting the interest group know via mailing list.