All posts by Felix Meyenhofer

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HRM update – new look, new features

We updated our Huygens Remote Manager to the version 3.7.0. New features include obviously the new look, file formats can be mixed now and there are some improvements on how the deconvolution templates can be configured. The full list can be found on the huygens-rm.org.

There is also a webinar about the new HRM 3.7 made by Daniel from SVI.

Finally here is a reminder about our own installation of the HRM. You can find our site specific documentation on our bioimage website:
http://www3.unifr.ch/bioimage/it/software-list/huygens-2/

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ImarisViewer – 3D visualisation utility

Oxford Instruments released the ImarisViewer. It is a subset of the Imaris functionality, essentially constraint to the 3D visualisation capabilities. But it a free version, that allows you browse your datasets you processed with Imaris.

Windows users being part of SECMED or BIOL can install the ImarisViewer via the Baramundi Kiosk.

Mac users can find the installer on our download page (visible only from within the UNIFR domain)

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Amira/Avizo – new software license

Our facility together with  several departments of the SCIMED faculty was able to acquire  licenses for Thermo Fisher’s 3D visualisation and image analysis software.

While Avizo is destined more for applications in the field of material sciences, Amira is targeted towards life sciences. The software also allows for third party extensions. A curated collection of such extensions is hosted by provider itself.

This type of software is particularly useful for very large datasets (big, possibly time-resolved volumes). This type of task also has high requirements concerning computer hardware. Therefore we installed in on our best image processing workstation at this time. This computer is bookable like a normal instrument.

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Huygens Professional

Our image processing workstation is now equipped with Huygens Professional (desktop). Compared to our Huygens Remote Manager (HRM) the HuPro allows for more flexibility and provides some additional functionality.

The new HuPro desktop version of the Huygens software is intended to accelerate the optimization of the deconvolution parameter and do advanced image restoration using measured point spread functions (in contrast to the theoretical PSF).

This new license replaces the Huygens Essential that was previously accessible via remote desktop connection.

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Imaris 9.3

Imaris 9.3 is available. Find out more about the software on the Bitplane website.

The first apparent change will be the end of the arena. Instead of its own database, there is now the possibility to add ‘observed’ folders to Imaris to facilitate the access to your ims-files.
The first time Imaris is launched, you will be prompted to use the migration assistant, that allows to extract existing images from the arena. You can find a short tutorial in this video.

You might also wonder look in vain for functionality like filtering or background subtraction under  Menu > Image Processing. Now instead there is a new icon in the toolbar:

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This will bring up the following window where you can chain together all the basic pre-processing operations together for the different channels of your dataset.

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The complete list of novelties can be found on the ‘what’s new‘ page. You might also be interested in the change-list in the release notes.

We installed this latest version on our number crunchers.

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FPbase

fpbase.org is a beautiful web-application to browse the contents of the Fluorescent Protein Database. There is a lot of linked content such as excitation and emission spectra or the publications reporting on the molecules. Check it out!

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Imaris Workshop 11. September 2018

At the University of Fribourg we use Imaris to process and analyse multidimensional image data-sets issue from epi-fluorescene microscopy.  In particular we use it’s advanced functionality for 3 dimensional (optically sectioned) image data and perform volumetric analysis.

We organise a worksop with representatives of Bitplane for you, to discover the possibilities of the software and train yourself as a user.

Further information:

Organisation

date: 11. September 2018 from 9:00-17:00
location: PER II, F230
participation conditions: UniFr staff, free of charge, registration required

Participants are free to only attend the presentation of Imaris or to work through the entire workshop.

Schedule

TIME DESCRIPTION
 09:00-10:30 presentation of Imaris (functionality, use cases)
 10:30-10:45   coffee break
 11:00-13:00 introduction to Imaris (basics)
13:00-14:00   lunch break
14:00-15:30 hands-on workshop
15:30-16:00   coffee break
16:00-17:30 hands-on workshop

 

Registration

registration closed.