The Leica STELLARIS 8 FALCON is a laser scanning confocal microscope capable of fluorescent lifetime imaging microscopy (FLIM).
Key features:
- Inverted DMI8 stand with motorized stage
- 8kHz tandem scanner
- Galvo z-stage
- 405nm Laser
- Pulsed white light laser (WLL) 440-790nm
- AOBS (acousto-optical beam splitter) as tunable dichroic
- 1 PMT (detector for transmitted light)
- 2 HyD S (hybrid detectors)
- 2 HyD X (hybrid detectors for FLIM)
- Environmental chamber with temperature and CO2 control
- Water pump for immersion objectives (20x, 40x)
Objectives
| correction | Mag. | NA | Immersion | Coverglass |
Working distance |
OTHER |
|---|---|---|---|---|---|---|
| Plan FLUOTAR | 5 | 0.15 | Dry | 0.17mm | 12mm | |
| Plan APO | 10 | 0.40 | Dry | 0.17mm | 2.2mm | |
| Plan APO | 20 | 0.75 | Oil, Glyc., Water | 0-0.17mm | 0.67mm (Water, 0.17mm cover slip) | Corr. CS2 |
| Plan APO | 40 | 1.10 | Water | 0.14-0.18mm | 0.65mn | Corr. CS2 |
| Plan APO | 63 | 1.3 | Glyc. | 0.14-0.19 | 0.30mm | Corr. CS2 |
Lasers
Diode laser 405 nm
Pulsed white light laser (WLL) 440-790nm. Pulse picker with 1nm steps. up to 8 lines at a time.
Filters (for binoculars)
The light source is a Leica LED 3.
| position | Filter cube | Excitation range | EmissionLight | Excitation filter | Dichromatic mirror | Suppression filter |
|---|---|---|---|---|---|---|
| 1 | LED 405 | UV | Blue | 405/60 | 455 | 470/40 |
| 2 | FITC | Blue | Green | 480/40 | 500 | 527/30 |
| 3 | EMPTY | - | - | - | - | - |
| 4 | RHOD | Green | Yellow | 546/10 | 560 | 585/40 |
Read up about filter-cubes.
Use the FPbase or the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.
Stage
XY-Table
| Property Name | Value |
|---|---|
| Reach | 127 x 83 mm |
| Speed | 240mm/s |
| Precision | +- 20 um |
| Repetition | < 5 um |
| Step size (resolution) | 0.7 um |
SuperZ Galvo
| Property Name | Value |
|---|---|
| Reach | 1.5 mm |
| Repetition | 49 nm |
| Step size (resolution) | 20 nm |