Leica STELLARIS 8 FALCON

leica stellaris 8 falcon

The Leica STELLARIS 8 FALCON is a laser scanning confocal microscope capable of fluorescent lifetime imaging microscopy (FLIM).

Key features:

  • Inverted DMI8 stand with motorized stage
  •  8kHz tandem scanner
  • Galvo z-stage
  • 405nm Laser
  • Pulsed white light laser (WLL) 440-790nm
  • AOBS (acousto-optical beam splitter) as tunable dichroic
  • 1  PMT (detector for transmitted light)
  • 2 HyD S (hybrid detectors)
  • 2 HyD X (hybrid detectors for FLIM)
  • Environmental chamber with temperature and CO2 control
  • Water pump for immersion objectives (20x, 40x)

Objectives

 correction Mag. NA Immersion Coverglass
Working distance
OTHER
Plan FLUOTAR 5 0.15 Dry 0.17mm 12mm
Plan APO 10 0.40 Dry 0.17mm 2.2mm
Plan APO 20 0.75 Oil, Glyc., Water 0-0.17mm 0.67mm (Water, 0.17mm cover slip) Corr. CS2
Plan APO 40 1.10 Water 0.14-0.18mm 0.65mn Corr. CS2
Plan APO 63 1.3 Glyc. 0.14-0.19 0.30mm Corr. CS2

 

Lasers

Diode laser 405 nm
Pulsed white light laser (WLL) 440-790nm. Pulse picker with 1nm steps. up to 8 lines at a time.

light spectrum

 

Filters (for binoculars)

The light source is a Leica LED 3.

position Filter cube Excitation range EmissionLight Excitation filter Dichromatic mirror Suppression filter
1 LED 405 UV Blue 405/60 455 470/40
2 FITC Blue Green 480/40 500 527/30
3 EMPTY -  - - - -
4 RHOD Green Yellow 546/10 560 585/40

Read up about filter-cubes.
Use the FPbase or the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.

Stage

 XY-Table

Property Name Value
Reach 127 x 83 mm
Speed 240mm/s
Precision +- 20 um
Repetition < 5 um
Step size (resolution) 0.7 um

SuperZ Galvo

Property Name Value
Reach 1.5 mm
Repetition 49 nm
Step size (resolution) 20 nm