The TCS SP5 is an upright confocal laser scanning microscope suitable for:
- Fixed tissue imaging
- Live cell imaging
- z-stack
- Time lapse
- Tile scan
- ROI scan
Key features
- Upright DM6000CS stand with motorised stage
- Super-z Galvo
- 3 PMT detectors, 1 transmitted light detector
- Tunable emission filters
- Widefield fluorescent cubes: GFP, Rhodamine, DAPI
- Contrast method: DIC
- EL6000 Light Source
- Software Leica LAS (Version 2.7.3)
Objectives
| FLATFIELD CORRECTION | ABERRATION CORRECTION | MAG. | NA | IMMERSION | COVERGLASS |
CONTRAST | OTHER |
|---|---|---|---|---|---|---|---|
| Plan | FLUOTAR | 10 | 0.3 | Dry | 0.17 | ||
| Plan | APO lambda blue | 20 | 0.7 | Glyc/Water | 0.17 | DIC C | Corr. lambda blue Corr. UV |
| Plan | APO | 40 | 1.3 | Oil | 0.17 | DIC D | Corr. CS Corr. UV |
| Plan | APO CS | 63 | 1.3 | Glyc | 0.14-0.20 | DIC D | Corr. UV |
| Plan | APO lambda blue | 63 | 1.3 | Water | 0.14-0.20 | DIC D | Corr. lambda blue Corr. UV |
The DIC is setup for the 20x and 63x
Lasers
Diode Laser: 405 nm
Argon Laser: 458nm, 476nm, 488nm, 514nm
Helium-Neon Laser: 543
Helium-Neon Laser: 633
Filters (observation with binocular)
| POSITION | FILTER CUBE | EXCITATION RANGE | EXcITATION FILTER | DICHROMATIC MIRROR | SUPPRESSION FILTER |
|---|---|---|---|---|---|
| 1 | I3 | Blue | BP 450-490 | 510 | LP 515 |
| 2 | N2.1 | Green | BP 515-560 | 580 | LP 590 |
| 3 | Ana | - | - | - | Analyzer |
| 4 | A | UV | BP 340-380 | 400 | LP 425 |
Read up about filter-cubes.
Use the FPbase or the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.
Motorized stage
Motorised Scanning stage for DM6000CS