The TCS SP5 is an inverted confocal laser scanning microscope suitable for:
- Live cell imaging
- Fixed tissue imaging
Key features:
- Inverted DMI6000 stand with motorized stage
- Tandem scanner: conventional galvo and resonant (high speed)
- Fast piezo focus
- AOBS (acousto-optical beam splitter) as tunable dichroic
- 3 PMT detectors with spectral detection, 1 transmitted light detector
- Wide-field fluorescence cubes: DAPI, GFP, Rhodamine, CFP/YFP
- Contrast methods: DIC
- Temperature controled incubator
- Local drug application system (OctaFlow, ALA Scientific Instruments)
- Software: Leica LAS
Objectives
| Flatfield correction | ABERRATION CORRECTION | Mag. | NA | Immersion | Coverglass |
CONTRAST | OTHER |
|---|---|---|---|---|---|---|---|
| Plan | FLUOTAR | 10 | 0.3 | Dry | 0.17 | PH1 | |
| Plan | APO | 20 | 0.7 | Dry | 0.17 | DIC C | Corr. CS Corr. UV |
| Plan | APO | 40 | 1.0 | Oil | 0.14-0.20 | DIC D | No UV correction! |
| Plan | APO | 40 | 1.3 | Oil | 0.17 | DIC D | Corr. CS Corr. UV |
| Plan | APO | 63 | 1.3 | Glyc | 0.17 | DIC D | Corr. CS Corr. UV |
The Phase contrast is currently only setup with the 10x.
The DIC is setup for the 20x, 40x and 63x
Lasers
Diode Laser 405 nm
Argon Laser: 458, 477, 488, 496, 514nm
DPSS 561 nm (Diode pumped solid state Laser)
HeNe 633 nm (Helium-Neon Laser)
Filters (observation with binocular)
The light source is a Leica EL6000 with a mercury metal halide bulb.
| position | Filter cube | Excitation range | EmissionLight | Excitation filter | Dichromatic mirror | Suppression filter |
|---|---|---|---|---|---|---|
| 1 | I3 | Blue | Green | BP 450-490 | 510 | LP 515 |
| 2 | N21 | Green | Red | BP 515-560 | 580 | LP 590 |
| 3 | ANT | - | - | - | Analyzer | |
| 4 | EMP | - | - | - | - | |
| 5 | A | UV | Blue (DAPI) | BP 340-380 | 400 | LP 425 |
| 6 | CFP | Violet/Blue | Cyan | BP 436/20 | 455 | BP480/40 |
Read up about filter-cubes.
Use the FPbase or the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.
Motorized Stage
Motorised Scanning stage for DMI 6000:
- Spindle pitch: 1mm
- Speed: 10mm/sec- Resolution: 0,02-0,04µ
- Reproducibility: < 1µm
- Accuracy: +/- 3µm