Leica TCS SP5 (Med)

Leica_TDC_SP5

The TCS SP5 is an inverted confocal laser scanning microscope suitable for:
- Live cell imaging
- Fixed tissue imaging

 

Key features:

- Inverted DMI6000 stand with motorized stage
- Tandem scanner: conventional galvo and resonant (high speed)
- Fast piezo focus
- AOBS (acousto-optical beam splitter) as tunable dichroic
- 3 PMT detectors with spectral detection, 1 transmitted light detector
- Wide-field fluorescence cubes: DAPI, GFP, Rhodamine, CFP/YFP
- Contrast methods: DIC
- Temperature controled incubator
- Local drug application system (OctaFlow, ALA Scientific Instruments)
- Software: Leica LAS

 

Objectives

Flatfield correction ABERRATION CORRECTION Mag. NA Immersion Coverglass
CONTRAST OTHER
Plan FLUOTAR 10 0.3 Dry 0.17 PH1
Plan APO 20 0.7 Dry 0.17 DIC C Corr. CS
Corr. UV
Plan APO 40 1.0 Oil 0.14-0.20 DIC D No UV correction!
Plan APO 40 1.3 Oil 0.17 DIC D Corr. CS
Corr. UV
Plan APO 63 1.3 Glyc 0.17 DIC D Corr. CS
Corr. UV

The Phase contrast is currently only setup with the 10x.
The DIC is setup for the 20x, 40x and 63x

Lasers

Diode Laser 405 nm
Argon Laser: 458, 477, 488, 496, 514nm
DPSS 561 nm (Diode pumped solid state Laser)
HeNe 633 nm (Helium-Neon Laser)

light spectrum

 

Filters (for observation with the eye piece)

The light source is a Leica EL6000 with a mercury metal halide bulb.

position Filter cube Excitation range EmissionLight Excitation filter Dichromatic mirror Suppression filter
1 I3 Blue Green BP 450-490 510 LP 515
2 N21 Green Red BP 515-560 580 LP 590
3 ANT - - - Analyzer
4 EMP - - - -
5 A UV Blue (DAPI) BP 340-380 400 LP 425
6 CFP Violet/Blue  Cyan BP 436/20 455 BP480/40

Read up about filter-cubes.
Use the FPbase or the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.

Motorized Stage

Motorised Scanning stage for DMI 6000:
- Spindle pitch: 1mm
- Speed: 10mm/sec- Resolution: 0,02-0,04µ
- Reproducibility: < 1µm
- Accuracy: +/- 3µm