Manual

General

  • The minimal delay between starting up  and shutting down the microscope is ~15 minutes! You also need to wait ~15 minutes before starting again after shut down.
  • If somebody uses the microscope after you, follow the standby protocol
  • Do not use your home folder (c:\users\<you login>) to save acquired data. Use D:\User Data\ for image data. Beware that this is merely a buffer and no place to store data for longterm. We clean image data regularly (to keep the backup to a reasonable size).
    Use \\home\<your login> which is mounted as drive U:\ for data you want to keep or put it on your external hard drive.

 

Start Protocol

  1. switch on the PC/Microscope button on the main switch board
  2. if needed, turn on the epifluorescence light source (for observation via eye-piece)  located on the computer table
  3. turn on the microscope controller (under the microscope table)
  4. switch on the Scanner Power button
  5. switch on the Laser Power button (turns on the laser fan)
  6. turn on the laser key
  7. on the computer open your session with your UNIFR login and password

 

Main switches
Leica TCS SP5 Light source for eye-observation Leica TCS-SP5 microscope controller
Epifluoresecnce light source
Microscope controller

 

Standby Protocol

If someone will use the microscope after you within the next 1-2 hours:

  • In the LAS AF software go to the configuration panel and decrease the laser power to 0%,  tick off the laser check boxes.
  • Turn the lasers off by using the key on the main switch board.
  • Exit the LAS AF software
  • Log off the computer
  • Clean the objectives

 

if you are taking a break:

  • In the LAS AF software go to the configuration panel and decrease the laser power to 0%.
  • Turn the lasers off by using the key on the main switch board.
  • lock the computer (Start  > Arrow next to Shut down > Lock)

 

Shut Down Protocol

make sure you transferred your data on a server usb-stick or somewhere else than the microscopes computer (user data gets erased from the hard-disk regularly). 

  • in the LAS AF configuration window, decrease the laser power to 0%
  • close the LAS AF software
  • shut down the computer
  • turn off the microscope
  • turn off the light source
  • switch off the PC/Microscope button
  • switch off the Scanner Power button
  • turn  the key to the position OFF
  • leave the Laser Power button on to allow the laser to cool down for at least  20 minutes
  • during that time:
    - clean up your stuff
    - clean the objectives
    - put the cover on the microscope
    - make sure the immersion liquid bottles are closed properly.
  • after ~15 minutes, when the laser fan shuts down (and you can hear yourself again), switch of the Laser Power button

 

Documentation

LASAF software documentation page

TCS SP5 system user manual

Leica DMI6000 manual