The DeltaVision Elite from Applied Precision is an inverted wide-filed fluorescence microscope for live cell imaging.
Key features
- Olympus IX71 inverted microscope
- CoolSnap HQ2 cooled CCD camera
- Fast excitation switching
- Laser module for TIRF/FRAP (The objectives currently only support the FRAP applicaiton)
- Motorized stage and focus
- Hardware based autofocus
- Incubation Chamber (C02, himidity and temperature control)
- On-site Deconvolution
Objectives
all objectives are from Olympus
| FLATFIELD CORRECTION | ABERRATION CORRECTION | MAG. | NA | IMMERSION | COVER GLASS |
DIC |
|---|---|---|---|---|---|---|
| U Plan | S Apo | 20 | 0.75 | air | 0.17 | - |
| U Plan | FL | 40 | 1.3 | oil | 0.17 | - |
| Plan | Apo N | 60 | 1.42 | oil | 0.17 | Turret 4 BFP1 |
| U Plan | S Apo | 100 | 1.40 | oil | 0.17 | Turret 5 Normal |
Lasers
Diode Laser: 405 nm (100mW), 488nm (50mW), 514nm (50mW), 561nm (50mW) e
The lasers can be used for FRAP. TIRF is not possible since we currently do not have the necessary objective.
Filters
Read up about filter-cubes.
Use the FPbase or the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.
Excitation
DAPI, FITC, TRITC, Cy5, POL
Emission
Blank, DAPI, FITC, TRITC, Cy5
Polychroic
(non motorized turret)
| POSITION | FILTER CUBE | Reflection bands |
Transmission bands |
|---|---|---|---|
| 1 | quad (DAPI/FITC/TRIC/Cy5) | 381-401, 464-492, 531-556, 619-644 |
409-456, 500-523, 564-611, 652-700 |
| 2 | quad-mch (DAPI/FITC/mCh/Cy5) | 381-401, 464-492, 561-590, 625-644 | 409-456, 500-553, 598-617, 652-700 |
| 3 | CFP/YFP | 400-454, 496-528 | 463-487, 537-559 |
| 4 | GFP/mCh | 425-495, 555-590 | 500-550, 600-675 |
| 5 | CFP/YFP/mCh | 400-454, 496-528, 558-593 | 463-487, 537-550, 602-648 |
Motorized stage
| Property | X,Y | Z |
|---|---|---|
| Accuracy | < 10.0 um per 25 mm | < 0.6 um per 13 um |
| Repeatability | < ± 0.2 um | < ± 0.1 um |
| Step resolution | 20 nm | 5 nm |
| Maximum travel | 25 mm x 50 mm | 1 mm |