Leica DMI6000B (Bio)

160119_Leica_DMI6000B

 

Key features:

- Inverted DMI6000 stand with motorized stage (only z-direction)
- Leica DFC360FX CCD camera (1.4M pixels, 20 fps)
- Ablation laser
- Fast filter wheels (for FRET)
- FRET Module
- EL6000 Light Source

Objectives

Flatfield correction ABERRATION CORRECTION Mag. NA Immersion Coverglass
CONTRAST OTHER
Plan FLUOTAR 5 0.15 Dry 0.17
Plan FLUOTAR 40 0.6 Dry 0.17
Plan APO 63 1.4-0.6 Oil 0.17 lambda blue
Plan APO 100 1.3 Oil 0.17 PH3

 

Lasers

NL100 Nitrogen laser 337nm (ablation)

Filters

Read up about filter-cubes.
Use the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.

Standard

position Filter cube Excitation range EmissionLight Excitation filter Dichromatic mirror Suppression filter
1 LAS 410
2 L5 Blue Green BP 480/40 505 527/30
3 RGR - - 495; 595
4 TX2 Green  Red 560/40 595 645/76
5 RCY 440; 520
6 ANT White  White

Fast

position Filter cube Excitation range EmissionLight Excitation filter Dichromatic mirror Suppression filter
1 EFIM
2 CYGmR-EX
3 CYGmR-EM - - 495; 595

 

Motorized Stage

Motorised Scanning stage (z-direction) for DMI 6000B:
- accuracy: ???

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