Leica DMI6000B (Bio)

160119_Leica_DMI6000B

Key features:

- Inverted DMI6000 stand with motorized stage (only z-direction)
- Leica DFC360FX CCD camera (1.4M pixels, 20 fps)
- Ablation laser
- Fast filter wheels (for FRET)
- FRET Module
- EL6000 Light Source

Objectives

Flatfield correction ABERRATION CORRECTION Mag. NA Immersion Coverglass
CONTRAST OTHER
Plan FLUOTAR 5 0.15 Dry 0.17
Plan FLUOTAR 40 0.6 Dry 0.17
Plan APO 63 1.4-0.6 Oil 0.17 lambda blue
Plan APO 100 1.3 Oil 0.17 PH3

Lasers

NL100 Nitrogen laser 337nm (ablation)

Filters

Read up about filter-cubes.
Use the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.

Standard

position Filter cube Excitation Emission Excitation filter Dichromatic Mirror Suppression filter
1 LAS 410
2 L5 460-500 512-542 BP 480/40 505 BP 527/30
3 RGR - - 495; 595
4 TX2 540-580  607-683 BP 560/40 595 BP 645/76
5 RCY 440; 520
6 ANT White  White

CFP/YFP/GFP/mRFP1 External Filter Set (Fast)

position CYGmR-EX CYGmR-EM Dichromatic Mirror
CFP BP 427/10 BP 472/30  CG1
YFP BP 504/12 BP 542/27  440/520
GFP BP 470/40 BP 520/40 YR2
RFP BP 572/35 BP 632/60 495/595

 

Motorized Stage

Motorised Scanning stage (z-direction) for DMI 6000B: