This microscope from Zeiss is an upright microscope equipped for:
- Stereology
- Brightfield (transmitted light)
- Fluorescence
Key features:
- Halogen lamp for transmitted light
- Halogen lamp for reflected light
- Fully motorized and computer controlled stage
- Monochrome high resolution camera (Hamamatsu Orca)
In particular this microscopes computer has the StereoInvestigator software installed stereology measurements of sections.
Objectives
| Flatfield correction | Aberration Correction | Magnification | NA | Immersion | Cover glass thickness | Special Property |
|---|---|---|---|---|---|---|
| Plane | Apochromat | 5x | 0.16 | air | 0.17 | |
| Plane | Apochromat | 10x | 0.32 | air | 0.17 | |
| Plane | Apochromat | 20x | 0.60 | water | 0.17 | |
| Plane | Apochromat | 40x | 1.00 | oil | 0.17 | Iris adjustment 0.5-1.0 |
| Plane | Apochromat | 63x | 1.40 | oil | 0.17 | |
| Plane | Apochromat | 100x | 1.30 | oil | 0.17 |
Filters
| position | Exitation filter | Dichromatic mirror | Emission filter |
|---|---|---|---|
| I | 450-490 | FT510 | 515-565 |
| II | BP546 | FT580 | LP590 |
| III | G265 | FT395 | LP420 |
| IV | 485/20, 578/14 | FT500, FT600 | 515-540, LP610 |
Camera
| manufacurer | model | resolution | pixelsize | objective | framerate | collector |
|---|---|---|---|---|---|---|
| Hamamatsu | Digital Camera ORCA-05G | 1344×1024 | 6.45×6.45 um | 0.6x | 8.9 | 0.25x |
Stage
BioPoint Screen 2 from Ludl Electronic Products Ltd. (LEP)
| Property Name | Property |
|---|---|
| Travel Range | 75mm x 50mm |
| Step Resolution | 0.025um |
| Position Repeatability | 2um |
| Motor Type | 0.9 degree stepper |
| Maximum Speed | 30mm/second |