Leica DM6000B (Med)

Leica_DM6000B

The Leica DM6000B is an automated upright microscope equipped for:
- Bright field (transmitted light)
- Fluorescence

 

Key features:

- Halogen lamp for transmitted light
- Mercury lamp for reflected light
- Fully motorized stage
- Hamamatsu monochrome camera

 

Objectives

Flatfield correction Aberration Correction Magnification NA Immersion Cover glass thickness  DIC Prism Special Property
plan S-APO 5x 0.15 air - C
plan FLUORETAR 10x 0.30 air - D
plan APO 20x 0.50 water - D U-V-I
plan APO 20x 0.70 Air/Glyc/Water - C Corr lambda BL
plan APO 40x 0.75 air 0.17 D PH2
plan APO 63x 1.20 Water 0.14-0.18 D Corr lambda BL

 

Filters

position Filter cube Excitation range Exitation filter Dichromatic mirror Suppression filter
1 A4  UV  BP 360/40 400 BP 470/40
2 L5 Blue BP 480/40  505 BP 527/30
3 Cy5 Red BP 620/60 655 BP 700/75
4 YFP Violet Blue BP 436/12; 500/20 445;515 BP 467/37;545/45
5 N2.1 Green  BP 512-560  580 LP 590

Read up about filter-cubes. The FluoScout or the Spectra-Viewer is a nice tool to plan filter and fluorophore usage.

Camera

manufacurer model resolution pixelsize objective
Hamamatsu C4742-95-12NR 1280×1024 6.7um 0.7x