Leica DM6B Navigator

leica_DM6B_0.411

This instrument is a fully automated wide-field microscope intended for imaging small series fixed samples and bigger tissue areas.

Location: PER02 0.410 ground floor

Key features

  • Upright microscope
  • Fully automated (stage, light path configurations, etc.)
  • High speed sCMOS
  • Color CMOS
  • LAS X Navigator (see screenshot bellow)
    setup high resolution image acquisition for slides, dishes and multi-well plates. Browse through the data with continuous zoom from the overview to the highest acquired resolution.

 

LAS X Navigator screenshot

Cameras

 Type  Model  Pixel Size Chip size Frame Rate Remarks
 sCMOS DFC9000 GT B/W  6.5um 2048 x 2048 50 frames/sec. 85% quantum efficiency
extended dynamic range of 33000:1
Color CMOS DMC2900  3.2µm 2048 x 1536 7.5 frames/sec. Dynamic range >55dB / 0:1

 

Objectives

All Objectives can be used with fully automated Nomarski DIC contrast

FLATFIELD CORRECTION Chromatic CORRECTION MAG. NA IMMERSION Functions
Plan FL 1.25 0.04 Dry
Plan FLUOTAR 10 0.32 Dry PH1, DIC (D)
Plan Apochromat 20 0.8 Dry PH2, DIC (C)
Plan Apochromat 40 0.95 Dry PH2, DIC (C)
Cover-slip 0.11-0.23mm
Plan Apochromat 63 1.4 Oil PH3, DIC (E)

Filter

This instrument has a hybrid filter system, consisting of filter cubes and filter wheels. The latter allow for fast switching of light path configuration, accelerating considerably multi-channel acquisitions.

Read up about filter-cubes.
Use the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.

Filter Turret

positon Name Notes Excitation Dichroic Supression
1 Ana DIC, PH
2 A UV BP 360/40 400 LP 425
3 I3 Blue BP 470/40 510 LP 515
4 CFT Quad DAPI/FITC/TRIC/Cy5
5 EMP Empty, BF

 Fast Filter Wheels

Name Notes Bands
DFTC-EX excitation filters DAPI BP 350/50x, FITC BP 490/20x, TRITC BP 555/25x, CY5 BP 645/30x, EMPTY
DFTC-EM emission filters DAPI ET 455/50m, FITC ET 525/36m, TRITC ET 605/52m, CY5 ET 705/72m, EMPTY

* Attenuation percentage

Stage

 Property Name  Value
Reach 225 x 76 mm
Speed 240mm/s
Repetition precision < 1 um
Step size (resolution) 0.01 um