This instrument is a fully automated wide-field microscope intended for imaging small series fixed samples and bigger tissue areas.
Location: PER02 1.402 first floor
Key features
- Upright microscope
- Fully automated (stage, light path configurations, etc.)
- High speed sCMOS
- Color CMOS
- LAS X Navigator (see screenshot bellow)
setup high resolution image acquisition for slides, dishes and multi-well plates. Browse through the data with continuous zoom from the overview to the highest acquired resolution.
Cameras
| Type | Model | Pixel Size | Chip size | Frame Rate | Remarks |
|---|---|---|---|---|---|
| sCMOS | DFC9000 GT B/W | 6.5um | 2048 x 2048 | 50 frames/sec. | 85% quantum efficiency |
| extended dynamic range of 33000:1 | |||||
| Color CMOS | DMC2900 | 3.2µm | 2048 x 1536 | 7.5 frames/sec. | Dynamic range >55dB / 0:1 |
Objectives
All Objectives can be used with fully automated Nomarski DIC contrast
| FLATFIELD CORRECTION | Chromatic CORRECTION | MAG. | NA | IMMERSION | Functions |
|---|---|---|---|---|---|
| Plan | FL | 1.25 | 0.04 | Dry | |
| Plan | FLUOTAR | 10 | 0.32 | Dry | PH1, DIC (D) |
| Plan | Apochromat | 20 | 0.8 | Dry | PH2, DIC (C) |
| Plan | Apochromat | 40 | 0.95 | Dry | PH2, DIC (C) Cover-slip 0.11-0.23mm |
| Plan | Apochromat | 63 | 1.4 | Oil | PH3, DIC (E) |
Filter
This instrument has a hybrid filter system, consisting of filter cubes and filter wheels. The latter allow for fast switching of light path configuration, accelerating considerably multi-channel acquisitions.
Read up about filter-cubes.
Use the FPbase or the Spectra-Viewer to analyse fluorophore spectra and microscopy configuration.
Filter Turret
| positon | Name | Notes | Excitation | Dichroic | Supression |
|---|---|---|---|---|---|
| 1 | Ana | DIC, PH | |||
| 2 | A | UV | BP 360/40 | 400 | LP 425 |
| 3 | CFT | Quad | DAPI/FITC/TRIC/Cy5 | ||
| 4 | I3 | Blue | BP 470/40 | 510 | LP 515 |
| 5 | EMP | Empty, BF |
Fast Filter Wheels
| Name | Notes | Bands |
|---|---|---|
| DFTC-EX | excitation filters | DAPI BP 350/50x, FITC BP 490/20x, TRITC BP 555/25x, CY5 BP 645/30x, EMPTY |
| DFTC-EM | emission filters | DAPI ET 455/50m, FITC ET 525/36m, TRITC ET 605/52m, CY5 ET 705/72m, EMPTY |
* Attenuation percentage
Stage
| Property Name | Value |
|---|---|
| Reach | 225 x 76 mm |
| Speed | 240mm/s |
| Repetition precision | < 1 um |
| Step size (resolution) | 0.01 um |