Leica DMRXA (Med)

Leica_DMRXA

Leica upright microscope equiped for:
- Imaging from fixed tissue: epifluorescence, DIC, dark-field

 

Key features:

- Epifluorescence: Dapi, Fitc, Rhodamine
- Dark-field
- DIC
- Color Camera (Spot Imaging Solutions)

 

Objectives

Flatfield correction Aberration Correction Magnification NA Immersion Cover glass thickness DIC Prism Special Properties
N Plan 2.5x 0.07 air - Î
PL FLUOTAR 5x 0.12 air - B
PL FLUOTAR 10x 0.30 air 0.17 A
PL APO 20x 0.60 air 0.17 B PH2
PL APO 40x 0.75 air 0.17 D
PL APO 63x 1.32-0.6 oil 0.17 D
PL APO 100x 1.40 oil 0.17 D

 

Filters

Position Filter cube Excitation range Excitation Dichroic Emission
1 I3 FITC BP 450-490 510 LP 515
2 EMPTY - - - -
3 N2.1 Rhodamine BP 515-560 580 LP 590
4 D DAPI BP 355-425 455 LP 470

Read up about filter-cubes. The FluoScout or the Spectra-Viewer is a nice tool to plan filter and fluorophore usage.

Camera

Manufacutrer Model Resolution Pixel Size Objective Condensor
Spot Imaging Solutions 14.2 color mosaic 2048×2048 7.4um x 7.4um 1x 1x