Light and fluorescence microscopy for Life Sciences
-
Teaching
Details
Faculty Faculty of Science and Medicine Domain Biology Code UE-SBL.00125 Languages English Type of lesson Lecture
Level Master Semester SA-2021 Schedules and rooms
Summary schedule Monday , Cours bloc (Autumn semester)
Contact's hours 28 Teaching
Responsibles - Egger Boris August
Teachers - Egger Boris August
- Lamy Christophe
- Meyenhofer Felix
- Schmidlin Eric
Description Fluorescence microscopy has become one of the core techniques in biological research. Its applications range from the study of the expression of specific molecular markers with high spatial resolution in single cells to the probing of cell functions in living organisms. Constant progress in microscope design and in fluorescent probe development has led to a large choice of applications based on the principles of fluorescence microscopy. This course will aim at giving an understanding of key concepts of the main techniques used in life sciences. It will also insist on practical issues essential for a productive use of these techniques in biological and biomedical research. Training objectives - The fundamental principles of light and fluorescence microscopy
- The differences between wide-field, confocal and 2-photon microscopy
- The basics of TIRF, FRET and FRAP
- The essential concepts in image acquisition and processing
- The basics of fluorescence imaging from live samples
- What types of fluorescent probes are available
- What super resolution and single molecule imaging can achieve
- How to image a whole organ at cellular resolutionCondition of access Seating will be assigned in order of registration, until the maximal capacity is reached.
Comments Moodle page: https://moodle.unifr.ch/course/view.php?id=260682
Softskills No Off field No BeNeFri Yes Mobility Yes UniPop No -
Dates and rooms
Date Hour Type of lesson Place 29.11.2021 08:15 - 12:00 Cours PER 21, Room B207 29.11.2021 13:15 - 17:00 Cours PER 17, Room 010 01.12.2021 08:15 - 12:00 Cours PER 21, Room F207 01.12.2021 13:15 - 17:00 Cours PER 17, Room 010 03.12.2021 08:15 - 12:00 Cours PER 21, Room B207 03.12.2021 13:15 - 17:00 Cours PER 17, Room 010 06.12.2021 08:15 - 12:00 Cours PER 21, Room B207 06.12.2021 13:15 - 17:00 Cours PER 17, Room 010 10.12.2021 08:15 - 17:00 Cours PER 21, Room D130 -
Assessments methods
Written exam - SA-2021, Session d'hiver 2022
Date 11.02.2022 13:15 - 14:00 Assessments methods By rating Descriptions of Exams Written exam (45 min.). One mark.
Written exam - SP-2022, Autumn Session 2022
Assessments methods By rating Descriptions of Exams Written exam (45 min.). One mark.
-
Assignment
Valid for the following curricula: Additional Courses in Sciences
Version: ens_compl_sciences
Paquet indépendant des branches > Specialized courses in Biology (Master level)
Additional programme requirements for PhD studies [PRE-DOC]
Version: 2020_1/v_01
Additional programme requirements for PhD studies (Faculty of Science and Medicine) > Specialized courses in Biology (Master level)
Biology [3e cycle]
Version: 2015_1/V_01
Continuing education > Specialized courses in Biology (Master level)
Biology [POST-DOC]
Version: 2015_1/V_01
Continuing education > Specialized courses in Biology (Master level)
MSc in Environmental Biology, option Applied Environmental Biology 120 [MA]
Version: 2022_1/V_01
MSc in Environmental Biology, option Applied Environmental Biology, lectures and seminars (from AS2021 on) > MSc-EB, option Applied Environmental Biology, elective courses (from AS2022 on) > Recommended courses
MSc in Environmental Biology, option Ecology and Evolution 120 [MA]
Version: 2022_1/V_01
MSc in Environmental Biology, option Ecology and Evolution, lectures and seminars (from AS2021 on) > MSc-EB, option Ecology and Evolution, elective courses (from AS2022 on) > elective courses
MSc in Environmental Biology, option Plant and Microbial Sciences 120 [MA]
Version: 2022_1/V_01
MSc in Environmental Biology, option Plant and Microbial Sciences, lectures and seminars (from AS2021 on) > MSc-EB, option Plant and Microbial Sciences, compulsory courses (from AS2022 on)
MSc in Environmental Biology, option Teaching 90 [MA]
Version: 2023_1/V_01
MSc in Environmental Biology, option Teaching, lectures and seminars (from AS2021 on) > MSc-EB, option Teaching, elective courses (from AS2023 on) > Recommended courses
MSc in Molecular Life and Health Sciences, option Biochemistry and Cell Biology 120 [MA]
Version: 2022_1/V_01
MSc in Molecular Life and Health Sciences, option Biochemistry and Cell Biology, lectures and seminars (from AS2021 on) > MSc-ML, option Biochemistry and Cell Biology, compulsory courses (from AS2021 on)
MSc in Molecular Life and Health Sciences, option Developmental Biology and Regeneration 120 [MA]
Version: 2022_1/V_01
MSc in Molecular Life and Health Sciences, option Developmental Biology and Regeneration, lectures and seminars (from AS2021 on) > MSc-ML, option Developmental Biology and Regeneration, compulsory courses (from AS2021 on)
MSc in Molecular Life and Health Sciences, option Marine Biology 120 [MA]
Version: 2022_1/V_01
MSc in Molecular Life and Health Sciences, option Marine Biology, lectures and seminars (from AS2021 on) > MSc-ML, option Marine Biology, compulsory courses (from AS2021 on)
MSc in Molecular Life and Health Sciences, option Neurobiology 120 [MA]
Version: 2022_1/V_01
MSc in Molecular Life and Health Sciences, option Neurobiology, lectures and seminars (from AS2021 on) > MSc-ML, option Neurobiology, compulsory courses (from AS2021 on)
MSc in Molecular Life and Health Sciences, option Teaching 90 [MA]
Version: 2023_1/V_01
MSc in Molecular Life and Health Sciences, option Teaching, lectures and seminars (from AS2021 on) > MSc-ML, option Teaching, elective courses (from AS2023 on) > Recommended courses