Details

Faculty	Faculty of Science and Medicine
Domain	Biology
Code	UE-SBL.00125
Languages	English
Type of lesson	Lecture
Level	Master
Semester	SA-2020

Schedules and rooms

Summary schedule	Monday , Cours bloc (Autumn semester)
Contact's hours	28

Teaching

Responsibles	Egger Boris August
Teachers	Egger Boris August Meyenhofer Felix Schmidlin Eric
Description	Fluorescence microscopy has become one of the core techniques in biological research. Its applications range from the study of the expression of specific molecular markers with high spatial resolution in single cells to the probing of cell functions in living organisms. Constant progress in microscope design and in fluorescent probe development has led to a large choice of applications based on the principles of fluorescence microscopy. This course will aim at giving an understanding of key concepts of the main techniques used in life sciences. It will also insist on practical issues essential for a productive use of these techniques in biological and biomedical research.
Training objectives	- The fundamental principles of light and fluorescence microscopy - The differences between wide-field, confocal and 2-photon microscopy - The basics of TIRF, FRET and FRAP  - The essential concepts in image acquisition and processing - The basics of fluorescence imaging from live samples - What types of fluorescent probes are available - What super resolution and single molecule imaging can achieve - How to image a whole organ at cellular resolution
Condition of access	Because of the current, exceptional situation, the number of participants is limited. Seating will be assigned in order of registration, until the maximal capacity is reached.
Softskills	No
Off field	No
BeNeFri	Yes
Mobility	Yes
UniPop	No

Written exam - SA-2020, Session d'hiver 2021

Date	16.02.2021 13:00 - 13:45
Assessments methods	By rating
Descriptions of Exams	Written exam (45 min.). One mark.
Comment	COVID-19 - Modalité d'évaluation: Written online exam 45 min. One mark.